Life on Earth originated in an intimate partnership between the nucleic acids (genetic instructions for all organisms) and small proteins called peptides, according to two new papers from biochemists and biologists at the University of North Carolina at Chapel Hill and the University of Auckland.
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Their "peptide-RNA" hypothesis contradicts the widely-held "RNA-world" hypothesis, which states that life originated from nucleic acids and only later evolved to include proteins.
The new papers - one in Molecular Biology and Evolution, the other in Biosystems - show how recent experimental studies of two enzyme superfamilies surmount the tough theoretical questions about how complex life emerged on Earth more than four billion years ago.
"Until now, it has been thought to be impossible to conduct experiments to penetrate the origins of genetics," said co-author Charles Carter, PhD, professor of biochemistry and biophysics at the UNC School of Medicine.
"But we have now shown that experimental results mesh beautifully with the 'peptide-RNA' theory, and so these experiments provide quite compelling answers to what happened at the beginning of life on Earth."
The special attributes of the ancestral versions of these enzyme superfamlies, and the self-reinforcing feedback system they would have formed with the first genes and proteins, would have kick-started early biology and driven the first life forms toward greater diversity and complexity, the researchers said.
Co-author Peter Wills, PhD, professor of physics at the University of Auckland, said, "Compared to the RNA-world hypothesis, what we've outlined is simply a much more probable scenario for the origin of life.
"We hope our data and the theory we've outlined in these papers will stimulate discussion and further research on questions relevant to the origins of life."
The two scientists are fully aware that the RNA-world hypothesis still dominates the origin-of-life research field. "That theory is so alluring and expedient that most people just don't think there's any alternative," Carter said. "But we are very confident there is."
Before there was life on Earth, there were simple chemicals. Somehow, they produced both amino acids and nucleotides that eventually became the proteins and nucleic acids necessary to create single cells. And the single cells became plants and animals.
Research this century has revealed how the primordial chemical soup created the building blocks of life. There is also widespread scientific consensus on the historical path by which cells evolved into plants and animals.
But it's still a mystery how the amino acid building blocks were first assembled according to coded nucleic acid templates into the proteins that formed the machinery of all cells.
The widely accepted RNA-world theory posits that RNA - the molecule that today plays roles in coding, regulating, and expressing genes - elevated itself from the primordial soup of amino acids and cosmic chemicals, eventually to give rise first to short proteins called peptides and then to single-celled organisms.
Carter and Wills argue that RNA could not kick-start this process alone because it lacks a property they call "reflexivity." It cannot enforce the rules by which it is made. RNA needed peptides to form the reflexive feedback loop necessary to lead eventually to life forms.
At the heart of the peptide-RNA theory are enzymes so ancient and important that their remnants are still found in all living cells and even in some sub-cellular structures, including mitochondria and viruses. There are 20 of these ancient enzymes called aminoacyl-tRNA synthetases (aaRSs).
Each of them recognizes one of the 20 amino acids that serve as the building blocks of proteins. Proteins, considered the machines of life, catalyze and synchronize the chemical reactions inside cells.
In modern organisms, an aaRS effectively links its assigned amino acid to an RNA string containing three nucleotides complementary to a similar string in the transcribed gene.
The aaRSs thus play a central role in converting genes into proteins, a process called translation that is essential for all life forms.
The 20 aaRS enzymes belong to two structurally distinct families, each with 10 aaRSs. Carter's recent experimental studies showed that the two small enzyme ancestors of these two families were encoded by opposite, complementary strands of the same small gene.
The simplicity of this arrangement, with its initial binary code of just two kinds of amino acids, suggests it occurred at the very dawn of biology.
Moreover, the tight, yin-yang interdependence of these two related but highly distinct enzymes would have stabilized early biology in a way that made inevitable the orderly diversification of life that followed. ■